Discussion & Conclusion
Based on the Reverse-Phase HPLC analysis to detect and quantify caffeine, the results obtained indicated that caffeine can still be found in Decaffeinated coffee.
Qualitative Analysis
Peaks that represent caffeine are present in decaffeinated coffee. These peaks occur at the same/similar retention time as the coffee. This is indicative of caffeine being one of the components in Decaffeinated coffee.
Quantitative Analysis
The various samples have relatively large signals produced, which fall within the linear dynamic range of the Standard calibration graph. (except Starbucks Decaf Coffee Sample, which has no peak). As such, the concentration of caffeine can be calculated in the various samples. This is then converted to the amount of caffeine found in a typical 8oz coffee. An average 8oz coffee contained approximately 95-135mg of caffeine. All of the coffee samples contains consistently lesser caffeine. However, the amount of caffeine present is not negligible.
Conclusion
Therefore, it is a false statement that decaffeinated coffee is caffeine-free. Coffee lovers should be aware of this and are discouraged to indulge in too much decaffeinated coffee with the misconception that it is caffeine-free. In particular, people with caffeine allergies should avoid decaffeinated coffee as there are still small amounts of caffeine present which can trigger allergic reactions. In conclusion, decaffeinated coffee is not free of caffeine. It is only coffee with lesser caffeine than in an average coffee mix. Over-consumption of decaffeinated coffee can still have detrimental effects on the body.
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